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Center for NanoFabrication








Cleaning procedures


Cleaning Procedures

Personal Actions Typically Prohibited in Cleanroom

  1. Fast motions such as running, walking fast or horseplay.
  2. Sitting or leaning on equipment or work surfaces.
  3. Writing on equipment or garments.
  4. Removal of items from beneath the cleanroom garments.
  5. Wearing the cleanroom garment outside the cleanroom.
  6. Wearing torn or soiled garments.

NO ONE who is physically ill, especially with respiratory or stomach disorders, may enter a sterile room.


General Cleanroom Regulations

Below is a list of general regulations recommended as a minimum for the successful operation of a cleanroom. All users and professional cleaning personnel should be aware and follow these regulations at all times.


  1. All personal items such as keys, watches, rings, matches, lighters and cigarettes should be stored in the personal locker outside the gowning room.
  2. Valuable personal items such as wallets may be permitted in the cleanroom provided they are NEVER removed from beneath the cleanroom garments.
  3. NO eating, smoking or gum chewing allowed inside the cleanroom.
  4. Only garments approved for the cleanroom should be worn when entering.
  5. NO cosmetics shall be worn in the cleanrooms. This includes: rouge, lipstick, eye shadow, eyebrow pencil, mascara, eye liner, false eye lashes, fingernail polish, hair spray, mousse, or the heavy use of aerosols, after shaves and perfumes.
  6. Only approved cleanroom paper shall be allowed in the cleanroom.
  7. Approved ball point pens shall be the only writing tool used.
  8. ses of paper or fabric towels are prohibited. Use of hand dryers equipped with HEPA filters is suggested.
  9. Gloves or finger cots should not be allowed to touch any item or surface that has not been thoroughly cleaned.
  10. Only approved gloves, finger cots (powder-free), pliers, tweezers should be used to handle product. Finger prints can be a major source of contamination on some products.
  11. Solvent contact with the bare skin should be avoided. They can remove skin oils and increase skin flaking.
  12. Approved skin lotions or lanolin based soaps are sometimes allowed. These can reduce skin flaking.
  13. All tools, containers and fixtures used in the cleaning process should be cleaned to the same degree as the cleanroom surfaces. All of these items are a source of contamination.
  14. NO tool should be allowed to rest on the surface of a bench or table. It should be place on a cleanroom wiper.
  15. Only cleanroom approved wipers are allowed to be used. The wipers must be approved for the Class of cleanroom being cleaned.
  16. s introduced into a sterile facility must be subjected to stringent sterilization prior to entrance.

Cleaning Procedures for a Class 1000 Section


Below is a cleaning program in a Class 1000 (Bio&Metrology) section of the NCS cleanroom.

Description of Work Frequency
Change tacky mats Every 2 hours
Wet mop with approved mop, cleaner & DI water 2 times per shift
Dust mop (if allowed) 2 times per shift
Remove trash, sweep, mop with appropriate cleaner wipe down tables and coffee area, clean walls and recycle cans 1 time per shift
Vacuum entry mats, sweep and mop floors 1 time per shift
Mop floor with pre-burnish cleaner and tap water 1 time per shift
Remove trash. Always wear gloves. Never take waste containers inside cleanrooms. 1 time per shift
Wet mop floors 1 time per shift
Remove acid and solvent trash 1 time per shift
Clean and replenish dispenser in all restrooms 3 times per week
Vacuum floor (if allowed) 2 times per week
Clean stainless steel pass throughs with s/s cleaner and appropriate wipes 1 time per week


Cleaning Procedures for a Class 100 Cleanroom

Procedure Frequency
Trash removal Once daily
Mop walkways Once a week
Wipe down horizontal surfaces Once monthly
Pull tacky mats Every 2 hours
Mop and trash removal Once daily
Wipe down walls and trim Once a week
Mop and trash removal Once daily
Wipe walls and trim Once a week
Mop Twice a shift
Wipe walls and trim Once a week
Vacuum Once monthly
Mop and trash removal Once per shift
Wipe down walls, windows, doors, trim, showers, passthroughs and fire extinguishers. Once a week