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Center for Life
Science
Micro Imaging
Genomics Unit
Flow Cytometry Unit
Protein preparation Unit
Media Preparation Unit
Histology Unit |
Center for Life Science consists of four units, suited on the first floor:
- Micro Imaging
- Biomedical Genomics, Transcriptomics, Proteomics and Metabolomics
Unit
- Flow Cytometry Unit
- Protein preparation Unit
- Media Preparation and Tissue Culture Unit
- Histology Unit
Micro Imaging
In addition to the Electron Microscopy facility located in Center for
Imaging, the Micro Imaging Unit will provide a imaging facilities including
optical and fluorescent microscopy, confocal microscopy, live cell imaging,
scanning and transmission electron microscopy,cryo-methods for tissue
preparation and digital imaging and image analysis. The core facilities:
- Confocal microscope
- MultiPhoton Confocal microscope for deep tissue imaging
- CW super-resolution confocal microscope
- High Pressure Freezer
- Cryo-ultramicrotome
Biomedical Genomics, Transcriptomics, Proteomics and Metabolomics Unit
The Biomedical Genomics, Transcriptomics, Proteomics and Metabolomics
Unit will provide a wide range of molecular biology technologies including
DNA sequencing, sequencing and electrophoresis, purification and
electrophoresis, electrophoretic separation, fragment analysis, DNA, RNA and
protein analysis, targeted genome and transcriptome, small RNA sequencing,
gene expression profiling, ChIP sequencing applications. The core equipment:
- Proteomics Discovery System
- Capillary LC and HCT Ultra Ion Trap
- Micro TOF/q and QQQ systems
- LTQ Linear Ion Trap Mass Spectrometers
- LTQ-Orbitrap Hybrid Mass Spectrometers
- Hybrid Mass Spectrometers
- Protein Sequencer
- Real-Time PCR System
- Fast Real-Time PCR System
- Microarraying Power Scanner
Flow Cytometry Unit
The Flow cytometry Unit will provide research technology platform in flow
cytometry, a high-speed laser technology for stem cell, cancer, immunology,
and neuroscience, research utilizing a variety of multi-laser cell sorters
and analyzers. It will provide the following services: cell sorting, cell
cycle analysis, apoptosis analysis, stem cell analysis. The core facilities:
- Avanti J-20 XPI – Beckman Coulter
- Cell sorter
- Flow cytometer
- Medimachine Sample PreparationSystems
- Bioimaging systems
- Laminar flow biosafety cabinets, refrigeration/freezer, cell
culture incubator, centrifugation, multi-channel pipettes
Protein preparation Unit
The Protein Preparation Unit will integrate with other units/services in
order to provide produce pure and active proteins for use in research
through some of the following services: protein purification, protein
Expression Optimization, etc. The core equipment:
- Express units
- Protein bioanalysers
- Liquid handling robot
- Bacteria/yeast growth facilities
Media Preparation and Tissue Culture Unit
The Media Preparation Unit will provide services related to preparation
of commonly used reagents and media necessary for their various research
applications and projects like (solutions, buffers, etc). The Tissue Culture
Unit caters for comprehensive tissue culture needs including cell banking,
transfection services (stable cell line production) and cell expansion
(mammalian and embryonic and custom stem cells). The core equipment:
- pH meters
- Beckman Coulter Vi-Cell XR
- incubators
- Bellco Roller Bottle incubators
- Microscopes
- EppendorfMultiporator
- Beckman Allegracentrifuges
- Multi gene thermocycler
- Shaking platforms
- Magnetic Stirrers
- Powers Scientific refrigerated incubator
- Precision refrigerated incubators
Histology Unit
The Histology Unit will provide a full-service in-house laboratory
dedicated to providing the highest quality histology services. The
technologies will provide for paraffin microtomy, cryotomy, vibratome
sectioning, plastic sectioning, and histochemical staining. It may also
include a repertoire of immunostaining protocols for adaptation to
individual antibodies and sample types. The core equipment:
- Automatic Tissue Processor
- Research Cryostat
- Automated Microtome
- Automated Vibratome
- Automated Cover Slipper Workstation
- Auto Stainer
- Microwave Retriever
- TBS Paraffin Embedding Center
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